1,Common observation of microscope-phase contrast (phase difference)
Phase-contrast microscopy utilizes the optical path difference of the specimen under examination. By exploiting the phenomenon of light interference, it converts phase differences imperceptible to the human eye into resolvable amplitude differences, making colorless and transparent objects clearly visible.
Phase contrast: The conversion of the optical path difference (i.e., phase difference) generated when light passes through the details of a transparent specimen into differences in brightness.
2, Common observation of microscope-phase contrast (phase difference)
Frog blood, positive phase contrast Frog blood, negative phase contrast
Positive phase contrast: Specimens with a refractive index higher than the surrounding medium appear as dark objects against a neutral gray background.
Positive phase contrast is applicable in a broader range of settings than negative phase contrast, and the image presentation of the observed target is more consistent with conventional viewing habits.
