The production of microscope biological slides can be divided into the following three steps:
Cleaning biological slides→ making smears→making slides.
- First, the biological microscope section cleaning.
The cleaning of microscope specimen section will directly affect the production quality, so the first step of making microscope specimen section is to clean the glass slide and cover glass.
The newly purchased biological slides should not be used directly. They should be soaked in 95% alcohol to remove the attachments on the surface, taken out before use, and dried or dried for later use.
The used old biological slides should be boiled in soapy water or washing powder for 0.5h, cooled, brushed with a soft brush to remove dirt, rinsed with tap water and put into 95% alcohol for later use. If it is a paraffin section, it can be soaked in xylene for several hours (waste xylene recovered during production can be used to avoid environmental pollution and save experimental cost), dissolve the gum used for sealing, separate the cover glass from the glass slide, and then boil it with soap.
- Second: a step of make a biological microscope slice.
1) The materials needed to make the biological microscope section are: cover glass, glass slide, dissecting needle, blade, writing brush, etc.
2) Take materials → Slice → Select slices → Fix and dye → Dehydrate → Transparent → Seal slices.
1. the materials are complete; The specimens should be fresh, and if the internal structure is eroded, the observation of the film will affect the integrity of the specimen structure.
2. slicing, slicing and materials should be stained with water from time to time; The action should be even and powerful, across the board, without cutting or stopping halfway.
3. After cutting the film, first put the slide in 30% alcohol water solution, let the tension of alcohol automatically unfold the wrinkles, and then move the slide to hot water, so that the film will be thinner; If the alcohol concentration is too high, it is easy to cause the glass slide to break and crack, and the tissues with less intercellular adhesion, such as fat, will disperse when they touch alcohol, so this method cannot be used.
4. Slides and coverslips must be cleaned.
5. Drop the prepared slices or the culture solution to be observed into distilled water.
6. Hold the cover glass and slowly put it down at an angle of about 45 degrees, so that the cover glass can cover the specimen without bubbles, and the excess water squeezed out can be sucked dry with blotting paper, so that it can be completed.
7. When sealing the film, the gum should be added in an appropriate amount, and it is advisable that the gum just spreads to its edge when the cover glass is covered; The concentration of gum should also be appropriate, and it should have a certain consistency and not be too thick, and it is advisable to slowly spread it under the cover glass.
