Phase Contrast Microscopy Systems

Product Overview

The Phase Contrast Microscopy System is engineered for high-contrast imaging of unstained, transparent specimens, including live cells, microorganisms, and thin tissue slices. By transforming phase shifts in light passing through a specimen into brightness changes, this system renders transparent structures visible without chemical fixation or staining. Built on a rigid die-cast aluminum frame with infinity-corrected optical pathways, it provides stable performance for continuous laboratory use, clinical diagnostics, and industrial quality inspection.

Technical Specifications

Technical Parameter	Specification Value
Optical System	Infinity-Corrected Optical System (f = 200 mm)
Viewing Head	Trinocular Siedentopf, Inclined 30 degrees, Interpupillary Distance 55-75 mm
Eyepieces	High-Eyepoint WF10x/22 mm with Diopter Adjustment
Phase Contrast Objectives	Infinity Plan Achromat Phase: 10x/0.25 (WD 5.0mm), 20x/0.40 (WD 2.0mm), 40x/0.65 (WD 0.5mm), 100x/1.25 Oil (WD 0.13mm)
Condenser	Turret Phase Contrast Condenser (BF, Phase Annuli for 10x-100x, Darkfield slot available)
Focusing Mechanism	Coaxial Coarse and Fine Adjustment, Tension Control, Minimum Fine Division: 0.001 mm
Stage	Double-Layer Mechanical Stage 185 x 142 mm, Movement Range 75 x 55 mm, Low-Position Right-Hand Control
Illumination	3W High-CRI LED (Color Temperature: 5500K) or 6V/30W Halogen, Pre-centered Lamphouse
Electrical Input	Universal Wide-Voltage Input: AC 100V-240V, 50/60 Hz; Regional detachable power cords (CE, UL/ETL, PSE certified)
Camera Port / Adapter	C-Mount Adapter (0.5x / 0.65x / 1.0x focus adjustable)

Key Features

Precise Phase Annulus Alignment

Independent centering screws on the turret condenser allow rapid, accurate matching of phase rings to objective focal planes, eliminating halo artifacts and uneven contrast.

Infinity-Corrected Optics

Delivers flat-field images across the entire 22 mm field of view, supporting modular integration of fluorescence or polarization attachments without optical degradation.

Rigid Structural Stability

The solid metal frame design minimizes mechanical vibration during high-magnification observation and motorized stage operations.

Thermal Management

LED illumination assembly features passive heat dissipation channels, preventing thermal drift during extended live-cell time-lapse sessions.

Applications

Live Cell Biology: Observation of cell morphology, division, and motility without fixation or fluorescent labeling.

Microbiology & Virology: Detection of bacterial spores, yeast, and unstained cellular inclusions.

Pharmaceutical QC: Contamination screening and particulate analysis in fluid samples.

Clinical Pathology: Routine urinalysis, sediment analysis, and unstained blood smear evaluation.

Configuration Options

Standard Configuration (Brightfield & Phase Contrast)

Trinocular Head + WF10x Eyepieces + 4x Phase Objectives + Turret Condenser + LED Illumination

Advanced Digital Configuration

Standard Configuration + 4K CMOS Scientific Camera + Measurement & Stitching Software Suite

Modular Upgrades

Fluorescence Module (LED Turret) / Polarization Kit / Heating Stage Inserts

OEM/ODM and Customization

Optical Customization

Objective thread pitch, custom magnification steps, and specialized filter sets (narrowband, UV/IR cut).

Mechanical Integration

Custom base dimensions, mounting interfaces for automated robotic handlers, and private-label housing aesthetics.

Manufacturing Capacity

CNC machining centers, cleanroom optical assembly benches, and automated laser interferometry testing ensure batch consistency for volume orders.

Quality Control

Interferometric Testing

Wavefront error and optical path difference measured for every objective batch using laser interferometers.

Alignment Calibration

Individual centering and phase ring concentricity verified on optical benches before packaging.

Environmental Stress Screening

Systems undergo temperature cycling (-10 degrees C to 50 degrees C) and drop-shock transit simulation conforming to ISTA standards.

Traceability

Each unit carries an engraved serial number linked to raw material test reports and individual optical QC certificates.

Packaging and Shipping

Packaging Structure: Custom-molded high-density EPE foam cradles enclosed in double-walled corrugated export cartons; wooden crating available for bulk freight.

Component Isolation: Optical heads, condensers, and objectives are packed in anti-static, hermetically sealed blisters to prevent dust and moisture ingress.

Logistics & Incoterms: Flexible shipping terms available including EXW, FOB, and DDP upon request. Global export compliance with regional electrical safety directives.

Frequently Asked Questions

Q: How do you prevent optical misalignment during international shipping?

A: Optical components vulnerable to shock-such as objectives and condensers-are removed, individually boxed, and secured within custom EPE foam inserts. The main body features a locked focusing block to prevent stage drift during transit.

Q: Can this microscope be upgraded to fluorescence imaging later?

A: Yes. The infinity-corrected optical path features a modular rear port that accepts a plug-and-play LED fluorescence epi-illuminator module without requiring factory realignment.

Q: What camera sensor resolution is recommended for phase contrast imaging?

A: For standard documentation, a 5-megapixel CMOS sensor (pixel size 2.4 microns) is sufficient. For publication-grade or quantitative image analysis involving deconvolution, a high-sensitivity scientific CMOS (sCMOS) camera with smaller pixel binning is recommended.

Q: Do you support OEM branding with custom housing colors and logos?

A: Yes. We offer OEM/ODM services including private-label badging, custom pantone color powder coating, and customized user manual documentation for batch orders exceeding 10 units.

Q: What is the typical lead time for standard versus customized orders?

A: Standard catalog configurations ship within 7 to 10 business days. Custom optical configurations or OEM production runs typically require 4 to 6 weeks depending on specification complexity.

Q: What quality certifications and electrical compliances do your systems hold?

A: Our production facilities operate under ISO 9001 quality management standards. Instruments carry CE, RoHS, and electrical safety compliance (UL/ETL and PSE-compatible power supplies) for export to global markets.

Request a Quote

To receive a formal quotation, volume pricing, or technical drawings for your specific laboratory or distribution requirements, please provide your specifications below:
Required Quantity:
Target Application: (e.g., Live cell imaging, routine lab, OEM integration)
Desired Illumination: (LED / Halogen)
Accessories Needed: (e.g., Scientific camera, fluorescence module, carrying case)
Destination Port / Country & Incoterms Preference:
[Submit RFQ / Contact Technical Sales]

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