How to calculate the magnification of a conventional microscope in a laboratory?

Mar 08, 2025 Leave a message

How to calculate the magnification of a conventional microscope in a laboratory?

 

Many laboratories are using microscopes, but they don't know the relevant professional knowledge of microscopes; they only know how to operate them, but they may not be very clear about some basic common sense. So today we will talk about how to calculate the magnification of microscopes.

Some people may say that this is not a very simple question, but it is actually a little complicated.

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First of all, let's take an example: when the magnification of the eyepiece of the stereoscopic microscope is 10 times, the zoom range of the zoom body is 0.7X-4.5X, and the additional objective lens is 2X. Then its optical magnification is 10 times 0.7 times 2, and the lowest magnification of this microscope is 14 times, and the maximum magnification is 10 times 4.5 times 2 equals 90 times, so the total optical magnification of this stereoscopic microscope is 14 times to 90 times. Of course, this is only the actual magnification of the microscope host. Next is the digital magnification of the microscope.

 

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For example, the size of the monitor is 17 inches, and 1/3 of the microscope camera is used, so the digital magnification of the microscope camera is 72 times compared with the table below. The formula for calculating the digital magnification of the microscope is based on the configuration of the stereoscopic microscope above; the zoom is 0.7X-4.5X, and the additional objective is 2X. The camera eyepiece is 1 (if the camera eyepiece has no multiple, it need not be added to the calculation). According to the formula: objective lens x camera eyepiece magnification x digital magnification, the minimum magnification of digital magnification is 0.7 times 2 times 1 times 72 equals 100.8 times, and the maximum magnification of digital magnification is 4.5 times 2 times 1 times 72 equals 648 times. Then the range of digital magnification is 100.8 times to 648 times.

In this case, two formulas will appear:

1. Total optical magnification = eyepiece magnification x objective magnification.

2. Digital total magnification = objective lens X camera eyepiece magnification X digital magnification

 

This formula is suitable for any microscope, whether it is a metallurgraphic microscope, a biological microscope, and so on.