How do I choose a microscope?

Aug 17, 2024 Leave a message

The question needs to be broken down into the following two questions:

First, what do I observe? Second, how do I observe?


Let's talk about the first question: What do I observe?


There are many different types of microscopes.
Slices, petri dishes, particles, and metals all need different microscopes to be observed. Many people will have a misunderstanding: expensive is good, but this is not right. Categories are not connected, and it may be somewhat difficult to access and very laborious. So what we have to do is buy the most suitable microscope, not the most expensive one.


A brief introduction to several categories of microscopes


1. Biological microscope: This type of microscope is specialized in looking at sliced cells that are invisible to the naked eye.
2. Stereo microscope: Use a microscope to magnify what the naked eye can see, which is continuous magnification.
3. Polarizing microscope:
This kind of microscope is mostly used to see crystals, powders, particles, and minerals, and if the item is transparent, it needs to use transparent reflection.
4. Metallographic microscope:
This type is mainly used to observe the metal surface and cutting surface. According to the different requirements of the detection object, the stereo microscope can also see.
5. Fluorescence microscope:
This one is for measuring luminescent substances, mainly depending on the wavelength and excitation group, as long as it meets the requirements.


The second question is, "How do I observe?"


This involves the price of the microscope and the corresponding advantages and disadvantages.
It really is to use the experiment, or daily observation of the specific observation, according to the above analysis to buy the microscope, and then according to the microscope operating instructions to observe.


1. Eyepiece: Monocular or binocular?
If it needs to be watched for a long time, it must be binocular, and myopia can also be seen.
2. Objective lens
Objective lenses have four multiples: 4X 10X 40X 100X 100 (usually oil lenses). 185 achromatic objective is a normal style, but 195 achromatic objective is the best.
3. Work stage/platform
Spring calipers, double-layer moving platform, X&Y translation handwheel-this is very standard equipment. Single-layer workstations are difficult to use.
4. Light Source
Natural light is difficult to dim at first, and the light source is unstable and too dark. It is best to use a continuously adjustable light source, and different specimens are suitable for different brightnesses. Halogen lamps are yellow, which are widely used in hospitals, and LED lamps are white, which are widely used in daily life.

 

Now, do you know how to choose a microscope after the above introduction?